This study was implemented to examine the effect of tobacco on operation of a retail store and propose a relevant direction to development of a retail store. In terms of an examination method, the questionnaire was conducted in respect of supermarkets and convenience stores(CVS) around the nation. As a result of analysis based on such questionnaire, although there was a little difference in between a supermarket and a convenience store, a similar result could be found in general. In other words, retailers selling tobacco regarded the tobacco as a medium to solicit customers. Since the significance of tobacco in terms of total sales volume was so high, without the tobacco, they might have suspend the business. Consequently, the effect of tobacco was enormous. In addition, under the premise that many regular customers should be acquired to develop such retail stores, it was found that it would be necessary to increase $15{sim}20%$ in terms of a margin in tobacco, preserve the profit ratio by the government, make efforts to enhance a quality and design by a tobacco company, actively recommend a particular brand, and actively display a tobacco publicity booklet, etc. Moreover, the proper number of tobacco stores in comparison with habitual smokers was one per 200 persons. In respect of the most unreasonable tobacco policy, a minor-related system and a policy of increasing a tobacco price were named. Thus, under the premise that a medium such as tobacco is highly important with respect to an operation of retail store, it is necessary to pay a more careful attention to an improvement of a reasonable and equitable system to further develop a retail store.