The objective of this study was to evaluate chemical water quality by hourly monitoring(25hr) of Saemangeum esturary. For this study, we selected 2 sites like a Mangyong Bridge(St. 6) and Dongjin Bridge(St. 7). Inflow of salt water was not detected during low tide(maximum 553, 508cm) of all stations, while the salinity rises were detected in spring tide(750cm). When 602m of maximum tide was reached, salinity concentration was increased at St. 7, while there was no change in St. 6. Therefore, We know that salinity variation is greatly influenced by tide height at survey site. Also, significant variance of salinity(p<0.05) was found between St. 6 and St. 7 because dike construction made the flood tide move into the Dongjin river. Total suspened solids(TSS) concentration was increased because of the river runoff at St. 6, and also the turbulance and resuspension according to salt intrusion at St. 7. During the high tide, the water discharge from the sea seemed to dilute the nutrient but to elevate TSS concentration in St. 7. Silicate and nitrate concentrations in the studied site were decreased by the mixing of sea water, whereas the evident trend of phosphate concentration was not found. This result can be explained by the phosphorus condition. Phosphorus exists inactive when it is affected by hydrated iron and adsorbed onto suspended matters. Compared to the environmental conditions of the St. 6 and St. 7, physical factors such as temperature, dissolved oxygen and TSS have statistically no significant difference(p<0.001), but nutrient concentrations were higher at St. 6 than St. 7. It could be suggested from these results that it is important to control the discharge of fresh water by sewage treatment plants located in St. 6 for water quality management.