An eSeal(Electrical Seal) is an active RFID device which installed on the door of a container. The main role of the tag is to make sure the seal is not breaking by unauthorized people. Because an eSeal uses RFID system, we need to prevent eavesdrop and impersonate. Moreover, an eSeal which uses a secure protocol must not be able to duplicate. If duplication resistant property is not provide to a eSeal, an attacker may replace a object in the sealed container and reseal the container with duplicated eseal to hide breaking of the eSeal. In this paper, we provide a protocol which resist duplicate an eSeal during prevent eavesdrop and impersonation.