The characteristics of non-point source pollutant loads in middle-stream of Nakdong River Were studied through analysis of pollutant loads of 8 sub-watersheds divided based on administrative district. The pollutant concentration of each sub-watershed was collected from Nakdong-River Water Research Institute and Daegu Regional Environmental Office, respectively. Pollution items analysed in this study were BOD, SS, T-N and T-P. High degree of non-point pollution was observed from Gumi, Goryeong, Gyeongsan, Daegu and Sungju, and pollution was usually increased during rainy season. This result indicates that a given pollution condition within the watershed can be more sensitive than location factor to the level of water quality. The main sources of non-point pollution were population and livestock, as well as landuse factor, and were found to significantly contribute to the water pollution. Alternative solutions for controlling pollution sources, therefore, should be provided to meet target levels of water quality in this region.