A closed type crankcase ventilation system has been adopted to engines to prevent emission of blow-by gas to atmosphere. In the early closed type crankcase ventilation system, blow-by gas which contains engine lubricating oil is re-circulated into the intake system. The blow-by gas containing oil mist leads to increased harmful emissions and engine problems. To reduce loss of the engine oil, a highly-efficient oil separation device is required. Principle of a cyclone oil separator is to utilize centrifugal force in the separator and, therefore, oil separator designs depend on rotational flow which causes the centrifugal force. In this paper, flow characteristics and oil separation performances for cyclone type designs are calculated with CFD methodology. In the CFD model, oil particle was injected on a inlet surface with Rosin-Rammler distribution and uniform distribution. The major design parameters considered in the analysis model are inlet area, cone length and outlet depth of the oil separator. As results, reducing inlet area and increasing cone length increase oil separation performance. Changes in outlet depth could avoid interference between rotational flow and outlet flow in the cyclone oil separator.