The purpose of this study is to verify availability of the acoustic emission in-situ monitoring method to the internal leak and operating conditions of the turbine major valves relating to safety for turbine operating and prevention of turbine trouble at nuclear power plants. In this study, acoustic emission tests are performed when the pressurized electro-hydraulic control oil flowed through turbine electro-hydraulic controller oil check valve and turbine power/trip fluid solenoid valve in the condition of actual turbine operating. The acoustic emission method was applied to the valves at the site, and the background noise was measured far the abnormal plant condition. To judge for the leak existence ell the object valves, voltage analysis and frequency analysis of acoustic signal emitted from infernal leak in the valve operating condition are performed. It was conformed that acoustic emission method could monitor for valve internal leak to high sensitivity