This study was performed to investigate the changes of the serological lipid-related parameters of the rats when they were fed with the high fat diets supplemented with or without rutin for five weeks. Twenty-four Sprague-Dawley male rats ($272.2{pm}7.2 g$ of body weight) were randomly divided into three groups: control (C) group and two treatment groups. Rats in the C group were fed with the high-fat diet containing 20% lard, 1% cholesterol and 0.5% sodium cholate (w/w) which was modified from the formula of the American Institute of Nutrition-76 (AIN-76) diet. Rats in treatment groups were fed with above diet supplemented with 0.75% rutin (R-0.75) or 1.5% rutin (R-1.5) on the weight to weight basis, respectively. The supplementation of rutin did not induce any significant difference on the final body weight, gain of body weight, the amount of feed intake and the feed efficiency of rats in both control and treatment groups. In addition the values of glucose concentration, total protein, albumin, globulin and albumin/globulin (A/G) ratio showed no significant differences among groups. The values of total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-C) in sera of rats in both R-0.75 and R-1.5 groups were lower than those in C group but the significances were showed in only between R-0.75 and C group (p < 0.05 and p < 0.01, respectively). The values of high density lipoprotein-cholesterol (HDL-C) in sera of rats in both R-0.75 and R-1.5 groups were higher than those in C group but the significances were showed in only between R-1.5 and C group (p < 0.01). The values of atherogenic index(AI) of rats in both R-0.75 and R-1.5 groups were the lower than those in C group (p < 0.01 and p < 0.05, respectively). The values of triglyceride in sera of rats showed no significant differences among groups. The values of AST and ALT in sera of rats showed no significant differences among groups. Therefore the supplementation of rutin to high fat diet in rats reduced effectively the serum lipid levels such as TC and LDL-C which were regarded as to cause the cardiovascular diseases, and moreover it elevated effectively HDL-C value which was regarded to protect cardiovascular diseases.