The fatigue life of ship structure under cyclic loading condition is made up of initiation and propagation stages. In this study, crack growth test is carried out on large scale structure test specimens and fracture mechanical analysis is performed. The fatigue lives measured from fatigue tests are compared with DNV, Matsuoka and BS 5400 S-N curve. And to predict the crack initiation life, S-N curve, corresponding to crack length 20mm at welded joint, is developed based on hot spot stress range. Also crack propagation life is calculated using crack growth equation. Consequently, computed crack propagation life is compared with experiment results.