The antioxidant activities, anti-inflammatory activity and cytotoxic effects of methanol extract from Euphorbia humifusa were evaluated in this study. Total phenolic compound contents were $68.35{pm}0.16$ mg/g and total flavonoid compound contents were estimated as $38.74{pm}1.26$ mg/g. EC50 values for DPPH radical scavenging activity of methanol extract was $56.26{pm}0.66{mu}g/mL$ and those of positive controls as ascorbic acid, ${alpha}$-tocopherol and BHA were $8.38{pm}0.14{mu}g/mL$, $16.45{pm}0.89{mu}g/mL$ and $21.18{pm}1.01{mu}g/mL$ respectively. NO scavenging activity increased in depending on concentration of extract. Treatment of RAW 264.7 cells with extract caused inhibition of LPS-induced nitric oxide production. The cell viability showed that the methanol extract had cytotoxicity in the growth of breast cancer cell ($66.54{pm}1.91%$ at $400{mu}g/mL$ conc., $43.98{pm}3.35%$ at $800{mu}g/mL$ conc.). Based on the results, It was suggested that the methanol extract of Euphorbia humifusa has a potential candidate for functional cosmetic and medicine.