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Effects of Sugar Type on Viability of Frozen-Thawed Canine Spermatozoa
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  • Effects of Sugar Type on Viability of Frozen-Thawed Canine Spermatozoa
  • Effects of Sugar Type on Viability of Frozen-Thawed Canine Spermatozoa
저자명
Lim. Y.H.,Son. J.M.,Shin. Y.J.,Kim. Y.S.,Lee. D.S.,Yoon. K.Y.,Shin. S.T.,Cho. J.K.
간행물명
韓國受精卵移植學會誌
권/호정보
2008년|23권 4호|pp.239-243 (5 pages)
발행정보
한국수정란이식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This study was conducted to investigate the effects of type of the sugar supplemented to the extender on the vigor, viability and intact acrosomal rates of frozen-thawed dog spermatozoa. The ejaculated semen was diluted with TRIS-citric acid extender containing 200mM TRIS, 73mM citric acid, 6% (v/v) glycerol, 20% (v/v) egg yolk, 1% (v/v) antibiotics (streptomycin/penicillin), 44 mM sugar, which was either glucose, fructose or glucose-fructose combination, and distilled water to make the final volume of 100ml. Extended semen samples were cooled at $4^{circ}C$ for an hour, packaged in 0.25ml straws, equilibrated for 10 minutes in liquid nitrogen vapor, and frozen in liquid nitrogen. Samples were thawed by placing straws into $37^{circ}C$ water for 120 seconds. After thawing, vigor, viability and intact acrosomal rates of frozen-thawed semen were compared according to type of sugar. No significant differences were observed between glucose and fructose groups. In addition, combination of the 2 sugars also did not show any significant differences in the vigor, viability and intact acrosomal rates. In conclusion, glucose and fructose were equally efficient as sugar supplements for freezing extender.