In this paper, we present a new image up-sampling method which registers low resolution images to the high resolution grid when Bayesian super-resolution image processing is performed. The proposed up-sampling method interpolates high-resolution pixels using high-frequency data lying in all the low resolution images, instead of up-sampling each low resolution image separately. The interpolation is based on B-spline non-uniform re-sampling, adjusted for the super-resolution image processing. The experimental results demonstrate the effects when different up-sampling methods generally used such as zero-padding or bilinear interpolation are applied to the super-resolution image reconstruction. Then, we show that the proposed hybird up-sampling method generates high-resolution images more accurately than conventional methods with quantitative and qualitative assess measures.