The effect of periodic blowing and suction of upstream flow on the separated shear flow behind the vertical fence was experimentally investigated. The fence was submerged in the turbulent shear flow and DPIV method was used to measure the instantaneous velocity fields around the fence. Periodic blowing and suction flow was precisely generated by the syringe pump. Spanwise nozzle made 2D planar periodic jet flow in front of the fence and the effect of frequency and maximum jet velocity was studied. From the results, the reattachment length can be reduced by 60% of uncontrolled fence case under the control.