Abstract - Objective: This study was conducted to investigate the effect of work conditions on job stress of social workers in Seoul. Method: For this survey, a self-reported questionnaire was administrated to 1,000 social workers working in all of organization for social welfare practice in Seoul. The number of responded questionnaires was 431. Multiple linear regression analysis was used for job stress as the dependent variables and control variables. Results: The result of multiple linear regression analysis indicated that regular rest breaks had significantly effect on job stress level but long working hours did not. When regular rest breaks was guaranteed job stress of social workers significantly lowered 8.4 point. In addition standardized regression coefficients and partial R2 of regular rest breaks was the highest score among the variables. Conclusion: This study suggests that it is the most important to guarantee regular rest breaks in the work schedule in order to alleviate job stress of social workers.