Many signal processing techniques have been described in the literature for estimating amplitude, frequency and duration variables of the surface EMG signal detected during constant voluntary contractions. They have been used in different application areas for the non-invasive assessment of muscle function. The main purpose of our research is to compare the most frequently used algorithms for information extraction from surface EMG signals under varying conditions in terms of the different window lengths, muscle contraction levels, muscles and subjects. In particular we focus on the issue of estimating the slope and intercept to resolve an linear regression line with utilizing real SEMG signals which represents voluntary contractions during thirty seconds.