본 연구는 고칼슘멸치가 흰쥐의 칼슘대사에 미치는 영향을 조사하기 위해 수행되었다. 실험처리는 시유와 칼슘강화우유, 칼슘강화소재(탄산칼슘, 젖산칼슘) 및 고칼슘대멸 등 5처리군으로 하였고, 대멸분말의 칼슘함량을 고려하여 최종식이의 칼슘함량은 1%가 되도록 semi-purified diet(AINdiet)에 혼합한 식이를 5주 동안 급여하였다. 대퇴골의 길이는 대조군인 우유군(M)와 칼슘강화우유군(M2)이 실험군 중 통계적으로 유의하게 길었고(p<0.05), BMD, BMC 및 칼슘함량은 고칼슘멸치군(MA)이 실험군 중 가장 높았다. 체외(in vitro) 칼슘흡수율은 고칼슘대멸군이 7.30%로 가장 높았고, 생체내(in vivo) 칼슘흡수율은 실험군간 차이는 없었지만 고칼슘대멸군이 27.50%로 가장 높았다. 혈청 중 총 콜레스테롤과 HDL-콜레스테롤 농도는 대조군과 고칼슘대멸군간에 통계적인 차이가 있었다(p<0.05). Creatinine 농도는 대조군, 고칼슘대멸군 및 탄산칼슘군이 칼슘강화우유군에 비해 통계적으로 높았다(p<0.05). 칼슘과 osteocalcin 농도 및 ALP활성은 실험군간에 차이가 없었지만 고칼슘대멸군이 높았다. SGOT활성은 칼슘강화우유군에 비해 대조군, 고칼슘대멸군 및 탄산칼슘군이 유의하게 높았다(p<0.05). 이상의 결과에서 고칼슘대멸군은 칼슘강화소재와 칼슘강화우유에 비해 대퇴골의 BMD, BMC 및 칼슘함량을 증가시켰으며, 생체내외(in vivo, in vitro) 칼슘흡수율에서도 다른 처리군보다 높았다. 본 결과는 대멸을 활용한 고칼슘식품 개발에 유용한 평가 자료로 활용될 것으로 사료된다.
This study was performed to investigate the effect of calcium-rich large anchovy on calcium metabolism in rats for 5 weeks. Experimental animals were randomly assigned to 5 treatments with 14 heads of Spraque Dawley male rats in each group. The experimental diets were as follows; market milk group (M) as control, market milk+calcium-rich large anchovy group (MA), market milk＋calcium carbonate group (MC), market milk+calcium lactate group (ML), and enriched-calcium market milk group (M2), which were formulated with commercially semi-purified rat chow (AIN-diet) to maintain the same level of calcium (1%) in all groups. Femur lengths of M and M2 groups were significantly higher than other groups. Bone mineral density (BMD) and bone mineral content (BMC) and calcium content of femur were the highest in MA group than other groups. In vitro and in vivo calcium absorption rates were high in MA group (7.30% vs 27.50%) compared with those of the other groups. Serum total-cholesterol and HDL-cholesterol levels were significantly different between M group and MA group (p<0.05). Creatinine levels were significantly higher in M, MA and MC groups than in M2 group (p<0.05). Serum calcium, osteocalcin and ALPase activities were higher in calcium-rich large anchovy (MA) group among the treatments, but there was no significant difference. SGOT activity was significantly lower in M2 group than those of M, MA and MC groups (p<0.05). These results may indicate that the calcium-rich large anchovy has enforced the BMD, BMC and calcium absorption rates of in vitro and in vivo compared with the other groups and might be a calcium-enriched food with large anchovy.
