Moisture damage of asphalt mixtures can occur because of a loss of bond between the asphalt binder and the fine and coarse aggregates. Therefore, moisture damage on asphalt pavements is the main cause of potholes, which is one of the main distress type of asphalt pavement. The purpose of this study is to evaluation effect moisture damage on material properties of asphalt mixtures through the laboratory performance test. The existing Modified Lottman test procedure was improved and the number of times that thermal cyclic conditioning can be added until the asphalt mixtures is damaged, was tested in order to exhibit the changes of the material properties because of moisture damage by immersion. Through the above experiments, it was found that the material properties of asphalt mixtures on room-temperature were rapidly decreased with loss of about 50% at initial stage of moisture damage caused by the amount of repeated immersion. Also, it was found that the property damage ratio using material properties of failure energy and $DCSE_f$ by test temperature $25^{circ}C$ were showed a high relationship to moisture damage of the asphalt mixtures caused by the amount of repeated immersion.