Image data produced from ADD water-tunnel test are currently analyzed manually. The accuracy and elapsed time of this process can be determined by observers. In this paper, the algorithm based on MATLAB for improved image data processing and analysis is proposed. This algorithm consists of camera calibration, gray-level transformation, noise filtering and binarization in image preprocessing, vortex trajectory measurement in image analysis. Experimental results show that the proposed algorithm has better accuracy and execution speed than those of the existing methods.