The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’to 29° 59’N latitude and 68° 5’to 68° 30’E longitude covering about 2360 km2 area and Dehradun district is situated between 77° 34’to 78° 18’E longitude and 29° 58’to 30° 58’N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.