The aim of this study is to evaluate the effects essential oil from Lavendular officinalis on the production of UVB-irradiated-induced nitric oxide(NO), in vivo and in vitro. NO is a recently discovered mediator of cell communication involved in a variety of physiological and pathophysiological processes. This enzyme is present in various tissues including smooth muscle cells and macrophages and take part in several immunopathological process. In vitro, the cytotoxicity and cell viability of aroma oil was evaluated by the MTT assay in the concentration of 0.01, 0.05, 0.1%. And, the effect of aroma oil was investigated to production of NO in human fibroblast cells line CCD-986sk ($2{ imes}10^5$ cell/well) after UVB-irradiation with aroma oil (0.01, 0.1, and 1%). The result showed that aroma oil did not affected the production of NO. In vivo, it was investigated to production of NO after UVB- irradiation with aroma oil. The experimental groups were divided into four groups. Aroma oil was stimulated the production of NO by itself. As the results, all of the in vitro and in vivo, aroma oil were affected production of NO by dependent the concentration-manners.