To evaluate the structural integrity of the NPP containment building more rigorously, the effective prestress, which is one of the most affecting elements, needs to be estimated exactly. This paper presents the results of an experimental study to determine the effective prestress force in prestressed concrete beams. It is possible to improve the effective prestress measuring method by test beam, which is being applied for the investigation of the nuclear power plant in operation. If experimentally evaluated Lift-Off method in this study can be coupled with test beam test currently being used in in-service nuclear power plant, it is possible to measure prestress loss of the tendon and the level of the effective prestress load.