As the concerns of dermal exposure of spray painters to isocyanates in the automobile industry, glove performance was examined like permeation rate and breakthrough time including fatigue test. Methylene chloride was used as the solvent for derivatization of the isocyanates with a 97.5% recovery. Ghost wipe pads were used to wipe the surface of the glove material after chemical penetration through the glove material placed under a disposable test cell. Several solvents were tested, such as thinner(xylene, toluene) and cleaning agent(acetone) by using a standard permeation test cell(AS/NZS standard 2161. part 10.3). Solvents accelerate chemical permeation through the gloves more quickly than pure HDI hardener products. The longest breakthrough times were from Nitrosolve gloves, not detected in 8 hours, compared with others like Latex, Neoprene, TNT and Dermo Plus. Therefore Nitrosolve gloves could be recommended as personal protective equipment in crash repair shops. In addition, revised exposure limit of korean regulation should be suggested for employee to minimize the risk of health symptoms.