This experiment states the ultra pure distilled water penetration depth through ray parenchyma cell in radial direction of Castanea crenata. Heartwood penetration depth was 1.16 times lower than the depth in softwood and that difference was found statistically significant at 75.2 second of penetration. Following go-stop-go cycle, water penetrated in the ray parenchyma cell. At the beginning this speed was high and then it was decreased slowly. Water penetration depth result was compared with alcohol penetration depth. It was found that water penetration in ray parenchyma was found lower than alcohol due to the low surface tension of water.