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서지반출
Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm
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  • Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm
  • Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm
저자명
Kang. Kyoung-Ho,Seon. Seung-Chun,Zhou. Bin
간행물명
한국패류학회지
권/호정보
2008년|24권 1호|pp.51-57 (7 pages)
발행정보
한국패류학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

With the purpose to estimate the possibility of short-term storage and cryopreservation for sperm of Charonia sauliae, which is a potential preparation for its artificial reproduction and further research, in this study, protocols for short-term storage and cryopreservation of trumpet shell sperm was optimized. The effects of different immobilizing solutions, dilution ratios were estimated for short-term storage. And the effects of different cryoprotectant extenders and freezing rates were estimated for cryopreservation in terms of motility and survival of sperm. The results indicated that the artificial sea water of 350 mOsmol/kg is a better immobilizing solution and sperm which was diluted at a ratio of 1:1 (v/v) had higher motility and survival rate during short-term storage. The effect of 5% dimethyl sulfoxide was significantly better than those of other cryoprotectant extenders. And a freezing rate of $-20^{circ}C;min^{-1}$ showed better effect than other freezing rates. In conclusion, this study optimized some key factors of the short-term and cryopreservation of C. sauliae sperm, which can provide valuable data for germ-plasm conservation and artificial propagation of C. sauliae.