This paper details a testing facility ("NATURAL FIRE FACILITY") that allows closely-controlled experimental testing on full-scale sub-frames while reproducing the spatially transient temperature conditions measured in real fires. Using this test facility, an experimental investigation of six steel sub-frames under a natural fire was carried out at the Department of Civil Engineering of the University of Coimbra. The main objective of these tests was to provide insight into the influence of these connection types on the behaviour of steel sub-structures under fire. The experimental layout is defined by two thermally insulated HEA300 columns and an unprotected IPE300 beam with 5.7 m span, supporting a composite concrete slab. Beam-to-column connections are representative of the most common joint type used on buildings: welded joints and extended, flush and partial depth plate. Finally, the available results are presented and discussed: evolution of the steel temperature; development of displacements and local deformations and failure modes on the joints zone.