To improve their growth and secondary metabolite production, we cultured Morinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm, as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or $45{mu}moL;m^{-2}s^{-1}$). Cell growth was greatest ($15.6g;L^{-1}$ dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids) was maximized at 0.1 vvm. A PPF of $15{mu}moL;m^{-2}s^{-1}$ accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF ($45{mu}moL;m^{-2}s^{-1}$) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of light on cell growth and secondary metabolite contents in M. citrifolia cell cultures.