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Succinic Acid Production by Continuous Fermentation Process Using Mannheimia succiniciproducens LPK7
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  • Succinic Acid Production by Continuous Fermentation Process Using Mannheimia succiniciproducens LPK7
저자명
Oh. In-Jae,Lee. Hye-Won,Park. Chul-Hwan,Lee. Sang-Yup,Lee. Jin-Won
간행물명
Journal of microbiology and biotechnology
권/호정보
2008년|18권 5호|pp.908-912 (5 pages)
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한국미생물생명공학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: ldhA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 was carried out at different glucose feed concentrations and dilution rates. After extensive fermentation experiments, a succinic acid yield and productivity of 0.38 mol/mol and 1.77 g/l/h, respectively, were achieved with a glucose feed concentration of 18.0 g/l and $0.2;h^{-1}$ dilution rate. A similar amount of succinic acid production was also produced in batch culture experiments. Therefore, these optimal conditions can be industrially applied for the continuous production of succinic acid. To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway.