The purpose of this study is to define optimal conditions for wool blend fabric dyeing using Rhus verniciflua extract. K/S value, surface color change, the fastness to light, fastness to dry-cleaning, metal mordanting effects and antimicrobial activities were investigated. The results were summarized as follows; As the storage time of Rhus verniciflua stokes dyeing solution was longer, K/S was improved. K/S value showed the highest one when dyeing temperature and time were at 100oC and 60 minutes respectively. As for the changes of pH values of the dyeing solution, fabric was dyed well with the pH value of acid or neutral. K/S value recorded the highest at pH6 and the lowest at pH10. Fastness to light showed the unmordanted fabric is 2.84, and Fe is 1.69 on color difference. Antibacterial activities of Rhus verniciflua dyeing solution recorded high figures in both dyed fabric with unmordanted and dyed fabric with mordanting.