With the recent rapid growth of RFID technologies, the Application of RFID systems into the medical or the military industries as well as into the distribution and logistics industry are now attempted continuously. The government and private sectors plan to carry out various small and large scale projects related to RFID systems. However, many companies attempting to apply RFID systems applications into their organizations are encountering many several difficulties because of the lack of installation experience and the absence of an useful guideline. This paper focuses on identification and classification of typical barriers to the successful application of RFID systems according to the five-step method of system application process. Moreover a "barrier map" is produced by conducting a survey and interviews by specialists. In addition, a practical guideline to overcome such barriers is presented and discussed.