Inactivation of Agrobacterium tumefaciens was evaluated on the inoculated fresh Radix Ginseng by electron beam irradiation or aqueous chlorine dioxide ($ClO_2$) treatment. Two groups of fresh ginsengs were prepared and inoculated with A. tumefaciens. One group was then irradiated at 0, 2, and 4 kGy using an electron beam accelerator, and the other group was treated with 0, 50, and 100 ppm of aqueous $ClO_2$. Microbiological data indicated that populations of A. tumefaciens significantly decreased with increasing irradiation dose or aqueous $ClO_2$ concentration. In particular, A. tumefaciens was eliminated by irradiation at 4 kGy, and 100 ppm $ClO_2$ treatment reduced the populations of A. tumefaciens by 1.44 log CFU/g. These results suggest that electron beam irradiation or aqueous $ClO_2$ treatment can be useful in improving the microbial safety of fresh ginsengs during storage.