본 연구는 시판되는 10종의 소독제(hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds(QAC), didecyl dimethyl ammonium chloride(DDAC), ortho-dichlorobenzene, and copper sulfate)에 대한 CHSE-214 cell line과 넙치 Paralichthys olivaceus, 조피볼락 Sebastes pachycephalus, 감성돔 Acanthopagrus schlegelii에 대한 24 h $LC_{50}$ 농도를 측정하였다. 시험에 사용된 시판용 소독제에 대한 CHSE-214 cell line의 24 h 독성 농도는 시판액을 기준으로 hydrogen peroxide 0.955 ppm, sodium hypochlorite 12.5 ppm, chlorine dioxide 62.5 ppm, povidon iodine 50 ppm, formaldehyde 4 ppm, glutaraldehyde 1 ppm, quaternary ammonium compounds 10 ppm, didecyl dimethyl ammonium chloride 0.4 ppm, ortho-dichlorobenzene 6.2 ppm 이었다. 10종의 소독제의 독성 농도는 어종에 뿐만 아니라 각 소독제에 따라서도 매우 다양하게 관찰되었다. Hydrogen peroxide는 소독제 중에서 가장 높은 농도에서 어류에 대한 독성을 보였는데 넙치, 조피볼락 및 감성돔에 각각 201, 269 및 139 ppm에서 $LD_{50}$을 나타내었다. DDAC는 가장 낮은 농도에서 어류에 대한 독성을 보였는데 넙치, 조피볼락 및 감성돔에서 각각 2.1, 1.0 및 1.5 ppm에서 $LD_{50}$을 나타내었다. Copper sulfate는 어종에 따라 독성을 보이는 농도가 가장 크게 나타났다.
Toxicity of ten commercial disinfectants(hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds(QAC), didecyl dimethyl ammonium chloride(DDAC), ortho-dichlorobenzene, and copper sulfate) was measured by chinook salmon embryo-214 cell line and three fish species: flounder Paralichthys olivaceus, black rockfish Sebastes pachycephalus, and black sea bream Acanthopagrus schlegelii. The $LC_{50}$ levels of 24 hours acute toxicity with the ten disinfectants were tested in three species of fish. Effectiveness of ten chemical disinfectants were varied by each chemical as well as by species. Hydrogen peroxide showed the higest activity at 201, 269, and 139 ppm in the flounder, the black rockfish, and black sea bream, respectively. DDAC showed the lowest activity at 2.1, 1.0, and 1.5 ppm in the flounder, the black rockfish, and black sea bream, respectively. The highest variation was observed in copper sulfate by both the chemicals and the species.
