The spray characteristics of a high pressure 6-hole injector were examined in a single cylinder optical direct injection spark ignition (DISI) engine. The effects of injection timing, in-cylinder charge motion, fuel injection pressure and coolant temperature were investigated using the 2-dimensional Mie scattering technique. It was confirmed that the in-cylinder charge motion played a major role in the fuel spray distribution during the induction stroke while the propagation of fuel spray was restrained during the compression stroke by the increasing pressure and the upward moving piston. In additions, it was confirmed that the liquid fuel droplets existing at the sprays edges were vaporized by the increase of the coolant temperature.