Purpose: To describe the knowledge and attitudes of nursing college students and non-nursing college students toward HIV/AIDS for use in the development of an effective HIV/AIDS education program. Method: The data were collected from 165 nursing college students and 92 non-nursing college students using a structured questionnaire, and then analyzed by $X^2$-test, t-test, ANOVA and Pearson correlation coefficient. Results: Compared to previous research, the score of some items of the knowledge and attitudes toward HIV/AIDS improved. However, the score of several items of the knowledge and attitudes toward HIV/AIDS needs further improvement. In both groups of college student, total attitudes toward HIV/AIDS were correlated with total knowledge of HIV/AIDS. Conclusion: Although some knowledge and attitudes toward HIV/AIDS improved comparing to previous research, a more specifically structured education program is needed. The development of a the program to increase the attitudes toward HIV/AIDS patients is critical.