In this study we try to identify the three-dimensional mixing characteristics of Seomjin River discharges in Seomjin River Estuary and Gwangyang Bay using a seasonal field observation (CTD) during spring tide and a three-dimensional numerical model with EFDC (Environmental Fluid Dynamics Code). The tidal elevation conditions of the four main tidal harmonic constituents on the open boundary and river discharges and thermal effluents at the specific boundary are considered. The calculated harmonic constants of tide and tidal current agreed well with those of observations at two stations for tide and two stations for tidal current. The model successfully reproduced well known the estuarine circulation in Seomjin River Estuary where tide and river discharges are dominant forcings. In the winter mean discharges case, tidal currents move Seomjin River discharges in Seomjin River mouth and in the summer mean discharges case, river flows move Seomjin River discharges near ae Seomjin River Estuary. A three-dimensional mixing characteristics of Seomjin River Estuary show well a three-dimensional estuarine circulation and thermal effluents effect to the seasonal variation of river discharges.