This paper aims to discussion the characteristics of location and spatial organization on the Donam SeoWon(confucian academy). To do this, we studied in analysis of the present conditions and photographing, research materials about the from of physical structure and review of historical documents. The result of analysis in the sight of the location and spatial organization was good matched. The spatial organization of the Donam SeoWon(confucian academy) was divided into entry space, lecturing space, management space and sacrificial space. Jonhakhumyo constructs axis of “Sanangru - Ipdeokmun - Yangseongdang - Naesammun - Sungryesa” and Eungdodang that not removed hall with 1880 shows characteristics a right angle in the left side of axis remove to location of present 1971. The result of this study, analysis of axis and Spatial structure shows backgrounds of these locations shows that the political and social, economical phenomena were influenced to the locations and spatial organization of the Donam SeoWon(confucian academy).