In this study, a hydrogenated lecithin-containing liposome was prepared using a sonicator and microfluidizer at high shear. The surface tension, particle size, turbidity, and transition temperature ($T_c$) of the liposome were investigated and compared with the commercially available standard surfactant Tween-60. The cytotoxicities were characterized using the MTT method. The surface tension of hydrogenated lecithin was found to be higher than that of Tween-60. The particle size prepared at above $T_c$ was smaller than that prepared below $T_c$. The results of the cytotoxicity experiment indicated that Lecinol 10 exhibits the highest I$C_{50}$ value, which shows its high safety in this study.