This study investigates the influence of slit positions on the microclimate temperature/humidity of garments. To design the slits, a market survey was performed to indicate the method to apply the slits, in addition to a literature survey about muscles and body surface variation through body movements. Based on the survey, three positions of slits were selected, shoulder slit, lowback slit, and midback slit, a slit width of 1cm and length to 30cm was used. The results showed that microclimate temperatures/humidity on the back according to the slit positions were in the order of, lowback, midback, and the shoulder. The lowback slit showed the highest effect on the temperature/humidity of a front trunk. Lowback slits affected on localized areas of the glutaeus maximus, erector spinae, and latissimus dorsi. Midback slits affected the back muscle and deltoid. Shoulder slits showed a more significant effect on the localized area of the deltoid versus other localized areas. In the subjective sensations, the lowback slit was cooler, dryer, and more comfortable than the other slits. For the subjective sensations by fabric characteristics, the slit positions correlated at |r|${geq}.8$ and were significant at p<.001 The results show that the lowback slit has a superior air exchange effect and thermoregulation qualities.