In this paper, the channel estimation and pilot sequence design technique of multiple-input multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) systems in multi-cell environments are studied for situations in which the inter cell interference (ICI) is the dominant channel impairment. We design pilot sequence aiming at minimizing mean square error and propose the channel estimation technique correspond to the designed pilot sequences. The proposed pilot sequences employ the sequences with good correlation properties such as Chu sequence and through simulations, it is shown that channel estimation algorithm using designed pilot sequence is effective for mitigating the ICI.