An in vivo model for assessing the efficacy of aspirin on the improvement of peripheral circulatory was developed by obtaining the cold-induced peripheral blood flow in mice using laser Doppler flowmetry (LDF) and temperature-controlled chambers. ICR mice (n=6) were placed in a temperature-controlled chamber and anesthetized with 1.5% isoflurane. LDF was used to measure the peripheral blood flow during whole-body warming for 10 min ($37^{circ}C$, baseline) and cooling for 40 min (until $13^{circ}C$). Peripheral blood flow ratio showed significant difference between baseline and whole-body cooling after a lapse of 20 ($0.92{pm}0.20$) and 40 min ($0.33{pm}0.13$, p<0.001). Peripheral blood flows of mice administered aspirin (100 mg/kg) or control substance (saline) were measured. Mean peripheral flow ratio decrement of the hind foot was significantly higher in aspirin ($0.23{pm}0.10$, p<0.05) than the control substance ($0.15{pm}0.04$). These findings indicate that wholebody cooling is characterized by peripheral circulatory disturbance, which also influences the efficacy of aspirin in improving the peripheral blood circulation.