We are developing the methods for the objective and systematic diagnosis, but in actuality the relativity between a diagnosis of Korean medical doctor to a symptom of patients and the conformity to the expression of the Korean medical diagnostic mechanism is short of the statistical data. so, the questionnaire of Cold-Heat & Deficiency-Excess and a diagnosis of Korean medical doctor and a result of the Korean medical diagnostic mechanism, through the relationship of those, we have offered the objective data for diagnostic validity. The study group was 750 volunteers who diagnosed by Cold-Heat & Deficiency-Excess, out of 1475 volunteers who participated in Korean-Western medical examination. We compared the results of the questionnaires for Cold-Heat & Deficiency-Excess patternization through the questionnaire with a diagnosis of Korean medical doctor. we also studied the diagnostic validity for the item of the questionnaire by statistics analysis. It is proper that 9 questions of 16 questions for the Cold, 6 questions of 14 questions for the Heat, 13 questions of 14 questions for the Deficiency, 6 questions of 9 questions for the Excess, and there is close correlation between the questionnaire to the diagnosis. The difference between the questionnaire score is meaningful(p=0.000), this conforms to the diagnosis of the Korean medical doctor, so the questionnaire have the validity. The result of the questionnaire of Cold-Heat & Deficiency-Excess conform to a diagnosis of Korean medical doctor, it carries an important meaning by the measure of diagnosis, and it is necessary for further study for the significance of the medical diagnostic mechanism.