A micro-mixer with a quasi-active rotor was fabricated, and mixing characteristics were evaluated. The proposed micro-mixer combines an active type micro-mixer with a passive type micro-mixer. The micro-rotor, which is a moving part of an active type micro-mixer, is added in a micro-chamber of a passive type vortex micro-mixer. The rotor rotated by inflows tangent to a chamber, causing strong perturbations. The micro-mixers were fabricated using photosensitive glass. Mixing efficiency of the micro-mixers was measured using an image analysis method. Mixing efficiency and characteristics of the micro-rotor mixer were compared with the vortex micro-mixer without a rotor. Mixing efficiency was reduced as Reynolds number increased at a low Reynolds number due to decrease of residence time. Mixing efficiency at higher Reynolds number, on the other hand, was improved even though residence time decreased since the contact surface between fluids increased by twisted flow. The perturbation induced by rotating rotor at greater than Re 200 improved the efficiency of the rotor mixer.