The principal objectives of this study were to evaluate the kinetics of lipase production by Staphylococcus wameri EX17 under different oxygen volumetric mass transfer coefficients ($k_{L}a$) and pH conditions in submerged bioreactors, using glycerol (a biodiesel by-product) as a carbon source. Cultivations were conducted at different $k_{L}a$ (26, 38, 50, and $83;h^{-1}$) and pH values (6.0, 7.0, and 8.0). The optimal $k_{L}a$ and pH were $38;h^{-1}$ and 7.0, respectively. Under these conditions, the maximal cell production obtained was 8.0 g/L, and the volumetric and specific lipase production reached high levels of activity, approximately 800 U/L and 150 U/g cell, respectively, after 12 h of cultivation. This result was approximately five times higher than that obtained in the shake flask cultures. The relationship between cell growth and lipase production was found to be associated with growth by the Luedeking-Piret model.