The accurate estimation of software reliability is important to a successful development in software engineering. Until recent days, the models using regression analysis based on statistical algorithm and machine learning method have been used. However, this paper estimates the software reliability using support vector regression, a sort of machine learning technique. Also, it finds the best set of optimized parameters applying immune algorithm, changing the number of generations, memory cells, and allele. The proposed IA-SVR model outperforms some recent results reported in the literature.