Periodic variation of local paper structure was evaluated using two-dimensional fast Fourier transform (FFT) and spectral analysis. Since the periodic variation could originate from various sources and have different magnitudes and patterns depending on the origins, a complete analysis of local paper structure properties such as local grammage, local thickness, local apparent density and surface topography was proposed in this study. For a commercial copy paper, the individual periodic patterns for each local structural property were identified by using inverse FFT spectrums of the filtered spectrum. The spectral analysis of newsprint sample provided the period of variation quantitatively, which was useful in comparing the origins of the individual periodic patterns of the local structural properties.