A Multilayered microelectrode design is presented for large area travelling wave dielectrophoresis (TwDEP) separators. Most of typical TwDEP chip has been arrayed with 1000 electrodes in $20{ imes}20;mm^2$. However, there is a limitation of the device area that is critical in throughput, because when the area of TwDEP becomes larger, the resistance of microelectrodes for bus bar is also increased. In this paper, we successfully developed a novel TwDEP chip with extremely large area ($31{ imes}25;mm^2$) by a unique multilayered bus bar design. According to the resistance simulation of our microelectrodes, it is possible to realize a TwDEP chip with an infinite longitudinal length. We demonstrated the feasibility of our suggestion with latex microbeads and showed the potential of extremely high throughput separation with TwDEP technique.