The most troublesome artifacts in micro computed tomography (micro-CT) are ring artifacts. The ring artifacts are caused by non-uniform sensitivity and defective pixels of the x-ray detector. These ring artifacts seriously degrade the quality of CT images. In flat-panel detector based micro-CT systems, the ring artifacts are hardly removed by conventional correction methods of digital radiography, because very small difference of detector pixel signals may make severe ring artifacts. This paper presents a novel method to remove ring artifacts in flat-panel detector based micro-CT systems. First, the bad lines of a sinogram which are caused by defective pixels of the detector are identified, and then, they are corrected using a cubic spline interpolation technique. Finally, a ring artifacts free image is reconstructed from the corrected projections. We applied the method to various kinds of objects and found that the image qualities were much improved.