Because steel girder bridge has big slenderness ratio, buckling is very important in design. Local buckling of plate girders having two longitudinal stiffeners in different positions under various load conditions is investigated. Various parametric study according to the change of web height, transverse stiffeners and load conditions are examined. These parametric studies are performed by numerical simulation utilizing finite element method. The objective of this study is to present the rational reinforcement location of two longitudinal stiffeners. The results of analysis are compared to that recommended by korean specifications for road bridges(2003).