Kinematic analysis of MTC (Muscle-Tendon Complex) units is a key indicator for diagnosis of patients with musculoskeletal disorders because the contracture or shortening of musculo-tendinous units is known to produce pathological gaits. Therefore, the principal objective of this study was to assess the length change in the triceps surae prior to and after wearing an AFO (Ankle-Foot Orthoses) in patients with musculoskeletal disorders during a gait. In this study, analyses were conducted using a Muscle Tendon Complex model coupled with the trajectory data from markers attached to anatomical landmarks. As a result, the maximum length change in the triceps surae during a gait was 4.87% when a barefoot walking group and a walking group with AFO were compared. In particular, the difference in length changes between both groups in Soleus MTC units was found to be statistically significant in all gait phases. Our results revealed that MTC length in the AFO walking group was clearly increased over that of the barefoot walking group. In the future, further studies will be required in order to more adequately assess musculoskeletal disorders using many cases studies with regard to agricultural working conditions because this study deals with the kinematic analysis of musculo-tendinous units in the case of clinical experiments.