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Brilliant Cresyl Blue 염색방법과 극체 방출 여부에 따른 돼지 체외수정용 난포란 선별 방법이 배발달에 미치는 영향
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  • Brilliant Cresyl Blue 염색방법과 극체 방출 여부에 따른 돼지 체외수정용 난포란 선별 방법이 배발달에 미치는 영향
  • Different Developmental Competence of Porcine Oocytes Selected by Brilliant Cresyl Blue Staining and Polar Body Extrusion
저자명
김연수,김철욱,김인철,곽대오,정기화,Kim. Yeon-Soo,Kim. Cheol-Wook,Kim. In-Cheol,Kwack. Dae-O,Chung. Ki-Hwa
간행물명
Reproductive & developmental biology
권/호정보
2009년|33권 1호|pp.29-33 (5 pages)
발행정보
한국동물번식학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The brilliant cresyl blue (BCB) has been used to select the developmental competent oocytes in pigs, goats and cows. Growing oocytes have a higher level of active glucose-6-phosphate dehydrogenase(G6PDH) compare to mature oocytes and are rarely stained compared to mature oocytes, because G6PDH converts BCB to colorless. First polar body extrusion regard as a guideline of meoisis completion. Selection of polar body extrude oocyte is more developmental competent to blastocyst than unselected. This study was conducted to compare the BCB test to the polar body extrusion on selection of developmental competent porcine oocytes for the production of blastocyst. Cumulus-Oocytes complex were exposed to 26uM BCB stain diluted in NCSU-23 for 90 min. There was no significant difference embryo development to blastocysts between BCB treated and not treated($19.58{pm}1.99$ vs $18.75{pm}2.27%$), which means there was no detrimental effect of BCB exposure to oocytes. Normal fertilization is not differed among treatment groups from 70.0 to 78.4% development to blastocyst, beside polyspermy did not. To compare two different selection methods, BCB test and polar body extrusion, evaluate the developmental competent of IVP embryos. BCB+PB+(blue stained and polar body extruded, $20.71{pm}0.45%$) and BCB-PB+(colorless and polar body extruded, $20.04{pm}l.29%$) groups are significantly (p<0.05) higher developed than those of BCB+PB-(blue stained and no polar body, $13.24{pm}0.73%$) and BCB-PB-(colorless and no poladbody, $7.25{pm}0.77%$). These results showed that selection of polar body extruded oocytes method is more efficient than that of BCB test.