This study was performed to elucidate the effects of addition of ${eta}-lactoglobulin$ and bovine serum albumin (BSA) in vitro maturation (IVM) and in vitro culture (IVC) medium on porcine embryo production. The development rate to the 2 cell ($71.4{sim}75.6%$) and blastocyst stages ($6.8{sim}13.3%$) with different BSA concentrations in IVM medium were similar among treatment groups. Blastocyst hatching rate was significantly higher in the control group (0.0mg/ml) than in the group of 1.0mg/ml supplement (20.0% vs. 0.0%; p<0.05). The development rate to the 2 cell ($62.0{sim}70.6%$) and blastocyst stages ($15.4{sim}38.5%$) with different ${eta}-lactoglobulin$ concentrations in IVM medium was similar among treatment groups. The development rate to the blastocyst was significantly higher in the group of 1.0mg/ml(15.3%) than in the group of 0.5mg/ml supplement (7.6%, p<0.05). The development rate to the 2 cell and blastocyst stages following the first addition of ${eta}-lactoglobulin$ in IVM medium was significantly higher in the control group (77.0% and 18.9%) and was $0{sim}44;hr$(77.2% and 16.9%) greater than that observed in other treatment groups (p<0.05). The development rate to the 2 cell stage ($68.1{sim}74.8%$) and blastocyst stages ($9.2{sim}12.7%$) with different BSA concentrations in IVC medium was similar among treatment groups. However, blastocyst hatching rate was significantly higher in the group of 3.0mg/ml supplement (30.0%) than in the control group (0.0%; p<0.05). The development rate to the 2 cell stage ($72.9{sim}78.0%$), blastocyst ($7.1{sim}14.2%$) and hatching stages ($33.3{sim}38.1%$) were not different. The development rate to the 2 cell stage ($63.6{sim}72.5%$), blastocyst ($8.4{sim}16.1%$) and hatching stages ($18.2{sim}37.5%$) at the different culture periods were similar among treatment groups. This study suggested that if the addition level and periods of ${eta}-lactoglobulin$ addition are adjusted, it is possible to replace BSA in the in vitro porcine embryo production.