본 논문은 한약복합물 HT008이 흰쥐 관절염 모델에 미치는 효과에 대한 것으로 주요 내용은 다음과 같다. Carrageenan에 의한 항부종 작용 실험은 흰쥐에 급성 관절염을 유발하기 위해 1% $lambda$-carrageenan을 투여하였고, 투여 전과 HT008 (10, 30, 100, and 300 mg/kg) 투여 후 1, 2, 4 및 6 시간에 각각 흰쥐 오른발 부피를 측정하였다. Acetic acid에 의한 진통 실험은 생쥐에 HT008을 300mg/kg 경구 투여한 후 30분 후에 0.7% acetic acid를 주사하고 10분 후부터 10분간 writhing syndrome 횟수를 측정하였다. 그 결과 HT008을 30, 100과 300 mg/kg 투여한 약재군은 부종이 감소하였다. 가시오가피와 황금 추출물 투여군은 대조군에 비해 유의한 통증 억제 효과를 확인하였다. 중국 당귀 추출물 투여군은 carrageenan으로 유발한 흰쥐 급성 관절염 모델에서 부종 억제 효과가 나타났다. 따라서 한약 혼합물 HT008은 관절염 모델에서 항염증 활성과 진통 효과가 있다는 내용이다.
Objectives : This study was conducted to investigate the effect of HT008, a multi-herbal mixture consisting of 3 herbs, Eleutherococcus senticosus, Angelica sinensis, and Scutellaria baicaleinsis on arthritic model in rats. Methods : The anti-inflammatory and analgesic activities were observed by utilizing the following models: carrageenan-induced edema of the hind paw of rats, acetic acid-induced writhing response in mice. The perimeter of the paw was measured before injection and then at 1, 2, 4, 6 h after injection of 1% $lambda$-carrageenan. The HT008 at five dose levels (10, 30, 100, and 300 mg/kg) and distilled water given 30 min to treatment groups and control group, before $lambda$-carrageenan injection. In the writhing test, the mice received 0.7% acetic acid solution in normal saline injected intraperitoneally at a dose of 10 ml/kg. The number of writhes was counted staring 10 min after injection. Results : HT008 at four dose levels (30, 100, and 300 mg/kg) significantly decreased the carrageenan-induced rat paw edema perimeter. E. senticosus and S. baicaleinsis extracts reduced acetic acid-induced writhing response in mice. Also A. sinensis extracts significantly decreased the carrageenan-induced rat paw edema perimeter. Conclusions : These results show that HT008, a multi-herbal mixture has both anti-inflammatory activity and analgesic effects in vivo arthritic model, and suggest that HT008 could be a good therapy to treat human osteoarthritis.
